Journal: Redox Biology
Article Title: SENP1-Sirt3 axis regulates type II alveolar epithelial cell activity to confer resistance against oxidative damage in lung tissue
doi: 10.1016/j.redox.2025.103752
Figure Lengend Snippet: Sirt3 KR mice reduce BLM-induced pulmonary inflammation and fibrosis . (A) Schematic of BLM challenge. (B) Survival curve (n = 5/group). (C) HE staining of mouse lung tissue sections. Scale bars, 100 μm. (D) Statistical analysis of cell number in BALF, each data point represents in an individual mouse (n = 3/group). (E) Statistical analysis of protein concentration in BALF, each data point represents in an individual mouse (n = 3/group). (F) Giemsa staining of BALF. Scale bars, 100 μm. (G) Masson staining of mouse lung tissue sections. Scale bars, 100 μm. (H) Ashcroft score analysis, each data point represents in an individual mouse (n = 3/group). (I) Western blot was used to detect the protein expression of collagen1 (Colla1) and α-SMA in the lung tissue, each lane represents in an individual mouse (n = 3/group). Data are presented as the means ± SEM. ∗∗p < 0.01.
Article Snippet: Giemsa staining of BALF was conducted according to the manufacturer's instructions (G1079, Servicebio).
Techniques: Staining, Protein Concentration, Western Blot, Expressing